Genetic variants of Interleukin 1-Beta-511 C>T and cervical intraepithelial neoplasia

I.C. Rotar, F.V. Stamatian, R. Popp, S.D. Bolboacă, F. Petrişor, D. Mureşan

Rezumat: Objectives. Interleukin 1β is a proinflammatory cytokine that plays a critical role in chronic inflammation and carcinogenesis. The aim of this study was to analyze the link between interleukin 1β promoter polymorphism -511 C>T, HPV infection and cervical intraepithelial neoplasia. Material and method. A case-control study was realized. The cases were represented by 128 patients diagnosed with cervical intraepithelial neoplasia, positive at HPV HR testing, while 111 controls, negative for intraepithelial neoplasia and negative at HPV HR testing, were included in the control group. Each person included in the study had a physical examination, cervical cytology, colposcopy, HPV HR testing, DNA extraction from peripheral blood and genotyping for -511 C>T SNP IL 1β using PCR RFLP technique. Results. For the case group, the absolute genotypes frequencies were as follows: 18 - C/C, 52 - C/T, 58 - T/T, respectively for controls: 14 - C/C, 50 - C/T, and 47 - T/T. Chi-square statistics had a value of 0.213, df=1, p=0.644 when the C/T&/T/T genotype was considered as a risk factor, respectively 0.108, df=1, p=0.743 when only homozygous TT genotype was taken into consideration as a risk factor. Odds ration had values of 1.128 (95% CI [0.676 - 1.884]) for C/T&T/T, respectively 0.882 (95% CI [0.417-1.867]) for T/Tgenotype. Conclusions. T/T genotype could be a protective factor for cervical cancer and no statistically significant link has been found between IL 1β-511 C>T and cervical intraepithelial neoplasia.
Cuvinte cheie: polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), Human Papilloma Virus (HPV), cervical intraepithelial neoplasia (CIN), interleukin 1β (IL-1β), single-nucleotide polymorphism (SNP)